The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of α-synuclein

Takafumi Hasegawa, Masatoshi Konno, Toru Baba, Naoto Sugeno, Akio Kikuchi, Michiko Kobayashi, Emiko Miura, Nobuyuki Tanaka, Keiichi Tamai, Katsutoshi Furukawa, Hiroyuki Arai, Fumiaki Mori, Koichi Wakabayashi, Masashi Aoki, Yasuto Itoyama, Atsushi Takeda

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105 Citations (Scopus)


Many neurodegenerative diseases share a common pathological feature: the deposition of amyloid-like fibrils composed of misfolded proteins. Emerging evidence suggests that these proteins may spread from cell-to-cell and encourage the propagation of neurodegeneration in a prion-like manner. Here, we demonstrated that α-synuclein (αSYN), a principal culprit for Lewy pathology in Parkinson's disease (PD), was present in endosomal compartments and detectably secreted into the extracellular milieu. Unlike prion protein, extracellular αSYN was mainly recovered in the supernatant fraction rather than in exosome-containing pellets from the neuronal culture medium and cerebrospinal fluid. Surprisingly, impaired biogenesis of multivesicular body (MVB), an organelle from which exosomes are derived, by dominant-negative mutant vacuolar protein sorting 4 (VPS4) not only interfered with lysosomal targeting of αSYN but facilitated αSYN secretion. The hypersecretion of αSYN in VPS4-defective cells was efficiently restored by the functional disruption of recycling endosome regulator Rab11a. Furthermore, both brainstem and cortical Lewy bodies in PD were found to be immunoreactive for VPS4. Thus, VPS4, a master regulator of MVB sorting, may serve as a determinant of lysosomal targeting or extracellular secretion of αSYN and thereby contribute to the intercellular propagation of Lewy pathology in PD.

Original languageEnglish
Article numbere29460
JournalPloS one
Issue number12
Publication statusPublished - 2011 Dec 22

ASJC Scopus subject areas

  • General


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