The β-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface

Koichi Tamano, Yuki Satoh, Tomoko Ishii, Yasunobu Terabayashi, Shinsaku Ohtaki, Motoaki Sano, Tadashi Takahashi, Yasuji Koyama, Osamu Mizutani, Keietsu Abe, Masayuki Machida

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The biological role of ExgA (Exg1), a secretory β-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA (exg1) gene were analyzed. The exgA disruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial growth rate and conidiation efficiency were observed for the exgA-overexpressing mutant than for the exgA disruptant when β-1,3-glucan was supplied as sole carbon source. On the other hand, no difference in phenotype was observed between them in the presence or absence of the inhibitors of cell wall β-glucan remodeling when grown with glucose. exgA Expression was induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression. These data suggest that ExgA plays a role in β-glucan utilization, but is not much involved in cell wall β-glucan remodeling.

Original languageEnglish
Pages (from-to)926-934
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number4
DOIs
Publication statusPublished - 2007 May 1

Keywords

  • Aspergillus oryzae
  • ExgA
  • Expression pattern
  • Glucan catabolization
  • β-1,3-exoglucanase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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