TY - JOUR
T1 - TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within the mouse testis
AU - Yoshitake, Hiroshi
AU - Tsukamoto, Hiroki
AU - Maruyama-Fukushima, Mayuko
AU - Takamori, Kenji
AU - Ogawa, Hideoki
AU - Araki, Yoshihiko
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for General Scientific Research, Nos. 18591813 and 1959192 and “High-Tech Research Center” Project for Private Universities: matching fund subsidy from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2008/7/25
Y1 - 2008/7/25
N2 - In adult male mice, the glycosylphosphatidyl inositol-anchored glycoprotein TEX101 is expressed only in germ cells and is thought to be involved in spermatogenesis. However, the details regarding the function of TEX101 remain to be clarified. We previously identified Ly6k as a candidate TEX101-associated protein, but as molecular probes are not currently available to detect Ly6k, we do not have conclusive evidence of the association between TEX101 and Ly6k. In this study, we confirmed the biological interaction between TEX101 and Ly6k using an established anti-mouse Ly6k polyclonal antibody (pAb). A combination of immunoprecipitation, Western blot, and immunohistochemical analyses using the pAb revealed that TEX101 is physically associated with Ly6k within the testis. In addition, these proteins simultaneously co-migrate into the detergent-resistant membrane fractions, suggesting that TEX101 collaborates with Ly6k on the cell membrane and may play a role in spermatogenesis.
AB - In adult male mice, the glycosylphosphatidyl inositol-anchored glycoprotein TEX101 is expressed only in germ cells and is thought to be involved in spermatogenesis. However, the details regarding the function of TEX101 remain to be clarified. We previously identified Ly6k as a candidate TEX101-associated protein, but as molecular probes are not currently available to detect Ly6k, we do not have conclusive evidence of the association between TEX101 and Ly6k. In this study, we confirmed the biological interaction between TEX101 and Ly6k using an established anti-mouse Ly6k polyclonal antibody (pAb). A combination of immunoprecipitation, Western blot, and immunohistochemical analyses using the pAb revealed that TEX101 is physically associated with Ly6k within the testis. In addition, these proteins simultaneously co-migrate into the detergent-resistant membrane fractions, suggesting that TEX101 collaborates with Ly6k on the cell membrane and may play a role in spermatogenesis.
KW - Detergent-resistant membrane fraction
KW - Glycosylphosphatidyl inositol-anchored protein
KW - Ly6k
KW - Spermatogenesis
KW - TEX101
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U2 - 10.1016/j.bbrc.2008.05.088
DO - 10.1016/j.bbrc.2008.05.088
M3 - Article
C2 - 18503752
AN - SCOPUS:44649108227
VL - 372
SP - 277
EP - 282
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -