TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within the mouse testis

Hiroshi Yoshitake, Hiroki Tsukamoto, Mayuko Maruyama-Fukushima, Kenji Takamori, Hideoki Ogawa, Yoshihiko Araki

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

In adult male mice, the glycosylphosphatidyl inositol-anchored glycoprotein TEX101 is expressed only in germ cells and is thought to be involved in spermatogenesis. However, the details regarding the function of TEX101 remain to be clarified. We previously identified Ly6k as a candidate TEX101-associated protein, but as molecular probes are not currently available to detect Ly6k, we do not have conclusive evidence of the association between TEX101 and Ly6k. In this study, we confirmed the biological interaction between TEX101 and Ly6k using an established anti-mouse Ly6k polyclonal antibody (pAb). A combination of immunoprecipitation, Western blot, and immunohistochemical analyses using the pAb revealed that TEX101 is physically associated with Ly6k within the testis. In addition, these proteins simultaneously co-migrate into the detergent-resistant membrane fractions, suggesting that TEX101 collaborates with Ly6k on the cell membrane and may play a role in spermatogenesis.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalBiochemical and biophysical research communications
Volume372
Issue number2
DOIs
Publication statusPublished - 2008 Jul 25

Keywords

  • Detergent-resistant membrane fraction
  • Glycosylphosphatidyl inositol-anchored protein
  • Ly6k
  • Spermatogenesis
  • TEX101

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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