TY - JOUR
T1 - Testis-specific expression of a novel mouse defensin-like gene, Tdl
AU - Yamamoto, Miwako
AU - Matsui, Yasuhisa
N1 - Funding Information:
We thank Dr Y. Nishimune for advice. This work was supported in part by the Science and Technology Agency of Japan, with Special Coordinating Funds for Promoting Science and Technology, by CREST of JST (Japan Science and Technology Corporation), and by grants-in-aid from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2002
Y1 - 2002
N2 - In order to isolate genes regulating sex differentiation of embryonic gonads, we have attempted to obtain genes specifically expressed in male embryonic gonads by means of subtractive hybridization screening, and we have cloned a novel mouse gene, Tdl. It potentially encodes a protein showing sequence homology to anti-microbial protein, β-defensins. Tdl reveals structural features such as the six cysteine residues with invariant spacing, which are found in β-defensins, but the overall amino acid similarity of Tdl to other members of the β-defensin family is low. In addition, the Tdl gene shows genomic organization similar to that of other β-defensin genes. We have found that Tdl is specifically expressed in Sertoli cell-lineage in seminiferous cords in embryonic testes, but not in embryonic ovaries after 12.5dpc when the sexual differentiation of gonads is initiated. Tdl is specifically expressed in gonads among adult tissues, and its expression persisted in Sertoli cells.
AB - In order to isolate genes regulating sex differentiation of embryonic gonads, we have attempted to obtain genes specifically expressed in male embryonic gonads by means of subtractive hybridization screening, and we have cloned a novel mouse gene, Tdl. It potentially encodes a protein showing sequence homology to anti-microbial protein, β-defensins. Tdl reveals structural features such as the six cysteine residues with invariant spacing, which are found in β-defensins, but the overall amino acid similarity of Tdl to other members of the β-defensin family is low. In addition, the Tdl gene shows genomic organization similar to that of other β-defensin genes. We have found that Tdl is specifically expressed in Sertoli cell-lineage in seminiferous cords in embryonic testes, but not in embryonic ovaries after 12.5dpc when the sexual differentiation of gonads is initiated. Tdl is specifically expressed in gonads among adult tissues, and its expression persisted in Sertoli cells.
KW - Sertoli cell
KW - Sex differentiation
KW - Subtraction cDNA screening
KW - Testis
KW - β-Defensin
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U2 - 10.1016/S0925-4773(02)00144-2
DO - 10.1016/S0925-4773(02)00144-2
M3 - Article
C2 - 12128228
AN - SCOPUS:0035997006
VL - 116
SP - 217
EP - 221
JO - Cells and Development
JF - Cells and Development
SN - 2667-291X
IS - 1-2
ER -