Tengunoibacter tsumagoiensis gen. nov., sp. nov., Dictyobacter kobayashii sp. nov., Dictyobacter alpinus sp. nov., and description of Dictyobacteraceae fam. nov. within the order Ktedonobacterales isolated from Tengu-no-mugimeshi, a soillike granular mass of micro-organisms, and emended descriptions of the genera Ktedonobacter and Dictyobacter

Chiung Mei Wang, Yu Zheng, Yasuteru Sakai, Atsushi Toyoda, Yohei Minakuchi, Keietsu Abe, Akira Yokota, Shuhei Yabe

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Three mesophilic, Gram-stain-positive, aerobic bacterial strains, designated Uno3T, Uno11T and Uno16T, were isolated from a soil-like granular micro-organism mass (termed Tengu-no-mugimeshi) collected from Tsumagoi, Gunma, Japan. They grow at 11-37 °C and pH 4.0-8.0, form branched mycelia, and have a G+C content between 49.4-50.3 mol%. The major menaquinone and fatty acid of Uno3T are MK-9 and iso-C16: 0, respectively, whereas Uno11T and Uno16T share MK-9 (H2) and C16: 1-2OH. The major cell-wall sugars are mannose (Uno3T and Uno11T) and glucose (Uno16T). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these three strains belong to the order Ktedonobacterales and are most closely related to Dictyobacter aurantiacus S-27T (sequence similarity of 91.3, 96.4 and 95.5%). Average nucleotide identity values were <79.9% among Uno11T, Uno16T and D. aurantiacus S-27T, well below the 95-96%species circumscription threshold. Based on phenotypic features and phylogenetic positions, we propose that Uno3T represents a novel genus and species, Tengunoibacter tsumagoiensis gen. nov., sp. nov. (type strain Uno3T=NBRC 113152T=LMG 30471T=BCRC 81113T) within the new family Dictyobacteraceae fam. nov. Strains Uno11T and Uno16T are also considered to represent novel species: Dictyobacter kobayashii sp. nov. (type strain Uno11T=NBRC 113153T=LMG 30472T=BCRC 81114T) and Dictyobacter alpinus sp. nov. (type strain Uno16T=NBRC 113154T=BCRC 81115T). We also propose an emended description of the genus Dictyobacter, classifying it within family Dictyobacteraceae, and provide emended descriptions of the genera Dictyobacter and Ktedonobacter.

Original languageEnglish
Pages (from-to)1910-1918
Number of pages9
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number7
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • Dictyobacteraceae
  • Ktedonobacteria
  • Tengu-no-mugimeshi

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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