Temperature-dependent ultrastructural changes in the cone interphotoreceptor matrix

Makoto Ishikawa, Toshiyuki Fujiwara, Takeshi Yoshitomi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Purpose: To determine the temperature-dependent ultrastructural changes of the cone matrix sheath, a possible critical factor in the adhesive strength binding photoreceptors and pigment epithelial cells. Methods: Aqueous-insoluble interphotoreceptor matrix (IPM) was extracted by soaking fresh bovine retinas in acidic HEPES-buffered solution followed by treatment with 1% Triton X-100 to remove cone membrane fragments. The IPM preparations were then incubated in the same buffer solution at either 34°C, 37°C, or 40°C for 60 min. After labeling with peanut agglutinin, the samples were prepared for electron microscopy. Results: The cone matrix sheath, specifically stained with peanut agglutinin, was markedly condensed at 34°C. It became more dispersed and composed of granular elements at 37°C. At 40°C, the matrix appeared as a network of fine filamentous structures. Conclusions: These findings suggest changing temperatures can alter the molecular organization of the IPM and thus influence its retinal adhesion.

Original languageEnglish
Pages (from-to)536-540
Number of pages5
JournalJapanese Journal of Ophthalmology
Volume53
Issue number5
DOIs
Publication statusPublished - 2009 Sep
Externally publishedYes

Keywords

  • Interphotoreceptor matrix
  • Retina
  • Temperature
  • Ultrastructure

ASJC Scopus subject areas

  • Ophthalmology

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