Temperature control for the expansion of artificial DNA motif

Yoshiharu Hatakeyama, Mitsuo Umetsu, Masahiro Minami, Satoshi Ohara, Seiichi Takami, Tadafumi Adschiri

Research output: Contribution to journalArticlepeer-review

Abstract

The DNA, which forms a double helical conformation by the highly selective base-pairing rule with the width of 2 nm, is one of the polymers utilized as a template for nanoparticle assembly. Then, holiday junction structure, where two different DNA double strands intersect and replace each complement strand, is an important artificial motif for forming two-dimensional mesh-like DNA morphology. In this study, the two-dimensional sheet with the mesh-like conformation is formed and the growth of the DNA morphology is controlled by changing the temperature in the formation process. The influence of the cooling procedure on the formation process of the two-dimensional DNA sheet is observed by atomic force microscope, and the formation of double strands in the process is traced by absorption spectra. The spectroscopic results demonstrate that the two-dimensional DNA sheet is strongly dependent on the correlation between the applied cooling process and the melting temperature of double helical conformation, and that the growth of the two-dimensional DNA sheet can be manipulated by the cooling procedure. Further, we will show the size control of the DNA sheet by mixing the designed DNA which inhibits the growth of the DNA sheet.

Original languageEnglish
Pages (from-to)2426-2430
Number of pages5
JournalJournal of Materials Science
Volume43
Issue number7
DOIs
Publication statusPublished - 2008 Apr 1

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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