Temperature changes in brown adipocytes detected with a bimaterial microcantilever

Masaaki K. Sato, Masaya Toda, Naoki Inomata, Hisataka Maruyama, Yuko Okamatsu-Ogura, Fumihito Arai, Takahito Ono, Akihiko Ishijima, Yuichi Inoue

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Mammalian cells must produce heat to maintain body temperature and support other biological activities. Methods to measure a cell's thermogenic ability by inserting a thermometer into the cell or measuring the rate of oxygen consumption in a closed vessel can disturb its natural state. Here, we developed a noninvasive system for measuring a cell's heat production with a bimaterial microcantilever. This method is suitable for investigating the heat-generating properties of cells in their native state, because changes in cell temperature can be measured from the bending of the microcantilever, without damaging the cell and restricting its supply of dissolved oxygen. Thus, we were able to measure increases in cell temperature of <1 K in a small number of murine brown adipocytes (n = 4-7 cells) stimulated with norepinephrine, and observed a slow increase in temperature over several hours. This long-term heat production suggests that, in addition to converting fatty acids into heat energy, brown adipocytes may also adjust protein expression to raise their own temperature, to generate more heat. We expect this bimaterial microcantilever system to prove useful for determining a cell's state by measuring thermal characteristics.

Original languageEnglish
Pages (from-to)2458-2464
Number of pages7
JournalBiophysical Journal
Volume106
Issue number11
DOIs
Publication statusPublished - 2014 Jun 3

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Temperature changes in brown adipocytes detected with a bimaterial microcantilever'. Together they form a unique fingerprint.

Cite this