Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein

Satoru Nagatoishi, Yoshikazu Tanaka, Motonori Kudou, Kouhei Tsumoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A structural polymorphism of nucleic acids, which depends on the concentration of cations and the conditions of hydration, are strongly involved with interactions between DNA and proteins. In this paper, we report that different DNA sequences bound to hyperthermostable TATA-box-binding protein (PhoTBP) at different combinations of temperature and salt concentration in in vitro selection experiments. As a result of the interaction of-these selected DNAs with PhoTBP, characteristic changes in the numbers of water molecules and ions occurred under each condition of the selection experiment. This finding could help us to understand the solvent environment-dependent preference for base sequences in protein-DNA interactions.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalMolecular BioSystems
Volume6
Issue number1
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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