Techno-functional properties and immunomodulatory potential of exopolysaccharide from Lactiplantibacillus plantarum MM89 isolated from human breast milk

Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Haruki Kitazawa, Francisco J. Barba, Laureline Berthelot, Muhammad Umair, Qinchang Zhu, Zhendan He, Liqing Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

An exopolysaccharide, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum MM89. It was comprised of glucose and mannose molecules with an average molecular weight of 138 kDa. FTIR and NMR spectra showed that MM89-EPS had characteristic polysaccharide functional groups. MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. To summarize, our results indicate that MM89-EPS can efficiently enhance the immunostimulatory activity of immune cells and an immunosuppressed mouse model. Hence, MM89-EPS may be use as a potential source of immunomodulatory agent in various food products.

Original languageEnglish
Article number131954
JournalFood Chemistry
Volume377
DOIs
Publication statusPublished - 2022 May 30

Keywords

  • Human breast milk, exopolysaccharide
  • Immunomodulatory activity
  • Lactiplantibacillus plantarum
  • Techno-functional properties

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Fingerprint

Dive into the research topics of 'Techno-functional properties and immunomodulatory potential of exopolysaccharide from Lactiplantibacillus plantarum MM89 isolated from human breast milk'. Together they form a unique fingerprint.

Cite this