Technique for high-quality protein crystal growth by control of subgrain formation under an external electric field

Haruhiko Koizumi, Satoshi Uda, Kozo Fujiwara, Masaru Tachibana, Kenichi Kojima, Jun Nozawa

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

X-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The average FWHMs for tetragonal HEW lysozyme crystals grown with an external electric field at 1 MHz were smaller than those for crystals grown without, especially for the 12 12 0 reflection. The crystal homogeneity of the tetragonal HEW lysozyme crystals was also improved under application of an external electric field at 1 MHz, compared to that without.Improvement of the crystal quality of tetragonal HEW lysozyme crystals grown under an applied field is discussed with a focus on subgrain formation. In addition, the origin of subgrain misorientation is also discussed with respect to the incorporation of impurities into protein crystals.

Original languageEnglish
Article number95
JournalCrystals
Volume6
Issue number8
DOIs
Publication statusPublished - 2016 Aug

Keywords

  • Crystal quality
  • Electric field
  • Protein crystals
  • Rocking curve

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Inorganic Chemistry

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