Technical communication Immunogold light and electron microscopic detection of amyloi plaques in transmissible spongiform encephalopathies

J. DOERR‐SCHOTT, T. KITAMOTO, J. TATEISHI, J. W. BOELLAARD, N. HELDT, C. LICHTE

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The antigenicity of the ‘prion’ protein amyloid fibrils was shown to be preserved after glutaraldehyde/OsO4 fixation in uranyl acetate‐ stained brain tissue blocks from patients with Gerstmann‐Straussler syndrome (GSS) and from mice infected with Creutzfeldt‐Jakob disease (CJD). Amyloid plaques were demonstrated by light microscopy in immunogold silver‐intensified semithin sections. Under the electron microscope, the amyloid fibrils were labelled in immunogold‐reacted ultrathin sections using an antiserum prepared against GSS amyloid plaque cores and mouse amyloid fibrils respectively. The influence of various oxidizing agents (hydrogen peroxide, sodium metaperiodate) on the tissue preservation and the immunohistochemical detection was tested.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalNeuropathology and Applied Neurobiology
Volume16
Issue number1
DOIs
Publication statusPublished - 1990 Feb
Externally publishedYes

Keywords

  • Gerstmann‐Sträussler syndrome
  • amyloid plaques
  • colloidal gold
  • immuno‐light and immuno‐electron microscopy
  • infected Creutzfeldt‐Jakob mouse
  • silver intensification
  • spongiform encephalopathies
  • ‘prion’ protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

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