Tauropine dehydrogenase from the sandworm Arabella iricolor (Polychaeta: Errantia): Purification and characterization

Nobuhiro Kanno, Minoru Sato, Eizou Nagahisa, Yoshikazu Sato

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


This is the first report of the purification of tauropine dehydrogenase (NAD: tauropine oxidoreductase) from a polychaete worm. In the sandworm Arabella iricolor Montagu (Polychaeta: Errantia), two forms of TaDH were detected: major component (pI = 7.5) and minor one (pI = 6.4). The major TaDH component was purified to homogeneity by means of (NH4)2SO4 precipitation, anion-exchange, affinity, chromatofocusing and hydrophobic chromatography, and characterized. From the molecular mass of 43.7 kDa obtained by rapid gel-filtration and that of 43.5 kDa by SDS-PAGE, the sandworm enzyme appeared to be a monomeric protein. Maximum rates of reduction of pyruvate and oxidation of tauropine were observed at pH 7.0 and 8.5, respectively. Pyruvate and taurine were preferred substrate for the enzyme. Apparent K(m) values determined using constant co-substrate concentrations were: 35.7 mM, 0.34 mM, and 0.036 mM for taurine, pyruvate and NADH, respectively, in the tauropine synthesizing reaction; and 4.8 mM and 0.051 mM for tauropine and NAD+, respectively, in the tauropine oxidizing reaction. The tauropine synthesizing reaction was subject to substrate inhibition by pyruvate: maximum rate was observed at 2.5-3.0 mM (inhibitory range of pyruvate concentration producing half-maximal rate was 26.8 mM).

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Issue number4
Publication statusPublished - 1996 Jan 1


  • Arabella iricolor
  • anaerobic glycolysis
  • opine
  • opine dehydrogenase
  • polychaete
  • pyruvate reductase
  • tauropine
  • tauropine dehydrogenase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Tauropine dehydrogenase from the sandworm Arabella iricolor (Polychaeta: Errantia): Purification and characterization'. Together they form a unique fingerprint.

Cite this