Tau 2: A Probe for a Ser Conformation in the Amino Terminus of τ

Nobuo Watanabe; Koji Takio; Masato Hasegawa; Takao Aral; Koiti Titani; Yasuo Ihara

doi:10.1111/j.1471-4159.1992.tb09349.x

Tau 2: A Probe for a Ser Conformation in the Amino Terminus of τ

Nobuo Watanabe, Koji Takio, Masato Hasegawa, Takao Aral, Koiti Titani, Yasuo Ihara

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala⁹⁵ through Ala¹⁰⁸ of bovine τ. Ser¹⁰¹ is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala⁹⁵ through Ala¹⁰⁸) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.

Original language	English
Pages (from-to)	960-966
Number of pages	7
Journal	Journal of Neurochemistry
Volume	58
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-4159.1992.tb09349.x
Publication status	Published - 1992 Mar

Keywords

Alzheimer's disease
Epitope mapping
Paired helical filaments–τ
Tau 2

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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title = "Tau 2: A Probe for a Ser Conformation in the Amino Terminus of τ",

abstract = "Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala95 through Ala108 of bovine τ. Ser101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala95 through Ala108) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.",

keywords = "Alzheimer's disease, Epitope mapping, Paired helical filaments–τ, Tau 2",

author = "Nobuo Watanabe and Koji Takio and Masato Hasegawa and Takao Aral and Koiti Titani and Yasuo Ihara",

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T2 - A Probe for a Ser Conformation in the Amino Terminus of τ

AU - Watanabe, Nobuo

AU - Takio, Koji

AU - Hasegawa, Masato

AU - Aral, Takao

AU - Titani, Koiti

AU - Ihara, Yasuo

PY - 1992/3

Y1 - 1992/3

N2 - Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala95 through Ala108 of bovine τ. Ser101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala95 through Ala108) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.

AB - Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala95 through Ala108 of bovine τ. Ser101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala95 through Ala108) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.

KW - Alzheimer's disease

KW - Epitope mapping

KW - Paired helical filaments–τ

KW - Tau 2

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