TY - JOUR
T1 - Tau 2
T2 - A Probe for a Ser Conformation in the Amino Terminus of τ
AU - Watanabe, Nobuo
AU - Takio, Koji
AU - Hasegawa, Masato
AU - Aral, Takao
AU - Titani, Koiti
AU - Ihara, Yasuo
PY - 1992/3
Y1 - 1992/3
N2 - Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala95 through Ala108 of bovine τ. Ser101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala95 through Ala108) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.
AB - Abstract: We have determined the epitope for Tau 2, a monoclonal antibody that intensely stained tangles, plaque neurites, and curly fibers in the tissue section, and strongly labeled bovine τ, but only very weakly labeled human r on the blot. The epitope has been localized to Ala95 through Ala108 of bovine τ. Ser101 is critical for Tau 2 reactivities; the replacement of Ser by Pro, which is found in rat, mouse, and human T, brings about very weak Tau 2 reactivities. The strong Tau 2 staining of tangles and its effective absorption with a synthetic Ser peptide (Ala95 through Ala108) suggest that the τ in paired helical filaments takes a Ser conformation, rather than a Pro conformation, in its ammo‐terminal portion.
KW - Alzheimer's disease
KW - Epitope mapping
KW - Paired helical filaments–τ
KW - Tau 2
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U2 - 10.1111/j.1471-4159.1992.tb09349.x
DO - 10.1111/j.1471-4159.1992.tb09349.x
M3 - Article
C2 - 1371153
AN - SCOPUS:0026507435
VL - 58
SP - 960
EP - 966
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 3
ER -