Targeting of apoptotic cells using functionalized Fe2O3 nanoparticles

Moataz Mekawy; Atsushi Saito; Hiroaki Shimizu; Teiji Tominaga

doi:10.3390/nano5020874

Targeting of apoptotic cells using functionalized Fe₂O₃ nanoparticles

Moataz Mekawy, Atsushi Saito, Hiroaki Shimizu, Teiji Tominaga

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Fe₂O₃ nanoparticles (NPs) have been synthesized and functionalized with SiO₂ and -NH₂ group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe₂O₃-SiO-NH₂ functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.

Original language	English
Pages (from-to)	874-884
Number of pages	11
Journal	Nanomaterials
Volume	5
Issue number	2
DOIs	https://doi.org/10.3390/nano5020874
Publication status	Published - 2015 May 26

Keywords

Apoptosis
Brain diagnosis
Fluorescently-labeled poly-caspase
Functionalized nanoparticles (FNPs)
Inhibitor (SR-FLIVO)

ASJC Scopus subject areas

Chemical Engineering(all)
Materials Science(all)

Access to Document

10.3390/nano5020874

Cite this

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title = "Targeting of apoptotic cells using functionalized Fe2O3 nanoparticles",

abstract = "Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.",

keywords = "Apoptosis, Brain diagnosis, Fluorescently-labeled poly-caspase, Functionalized nanoparticles (FNPs), Inhibitor (SR-FLIVO)",

author = "Moataz Mekawy and Atsushi Saito and Hiroaki Shimizu and Teiji Tominaga",

note = "Publisher Copyright: {\textcopyright} 2015 by the authors.",

year = "2015",

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doi = "10.3390/nano5020874",

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AU - Mekawy, Moataz

AU - Saito, Atsushi

AU - Shimizu, Hiroaki

AU - Tominaga, Teiji

PY - 2015/5/26

Y1 - 2015/5/26

N2 - Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.

AB - Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.

KW - Apoptosis

KW - Brain diagnosis

KW - Fluorescently-labeled poly-caspase

KW - Functionalized nanoparticles (FNPs)

KW - Inhibitor (SR-FLIVO)

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