Targeting of apoptotic cells using functionalized Fe2O3 nanoparticles

Moataz Mekawy, Atsushi Saito, Hiroaki Shimizu, Teiji Tominaga

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.

Original languageEnglish
Pages (from-to)874-884
Number of pages11
JournalNanomaterials
Volume5
Issue number2
DOIs
Publication statusPublished - 2015 May 26

Keywords

  • Apoptosis
  • Brain diagnosis
  • Fluorescently-labeled poly-caspase
  • Functionalized nanoparticles (FNPs)
  • Inhibitor (SR-FLIVO)

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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