Abstract
Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis.
Original language | English |
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Pages (from-to) | 874-884 |
Number of pages | 11 |
Journal | Nanomaterials |
Volume | 5 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2015 May 26 |
Keywords
- Apoptosis
- Brain diagnosis
- Fluorescently-labeled poly-caspase
- Functionalized nanoparticles (FNPs)
- Inhibitor (SR-FLIVO)
ASJC Scopus subject areas
- Chemical Engineering(all)
- Materials Science(all)