Targeting disruption of histamine H1 receptors in mice: Behavioral and neurochemical characterization

Kazuhiko Yanai, Li Zi Son, Masatosi Endou, Eiko Sakurai, Takehiko Watanabe

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

With gene targeting, one can practically knock out a gene in vivo and create a mutant organism that completely lacks the gene product. The mutant mice lacking histamine H1 receptors was generated by the method of gene targeting. In brains of homozygous mutant mice, no specific binding of [3H]pyrilamine was seen. The mutant mice showed impaired locomotor activity and exploratory behavior in an open field and activity wheel. Behaviors of the mutant mice were examined with several other tasks such as passive avoidance test, resident-intruder aggression test and formalin test to clarify the role for the H1 receptors in behaviors. Behavioral changes observed in the mutant mice are almost compatible with those obtained by the classical pharmacological tools. In correlation to the behavioral changes in the mutant mice, 5-hydroxytryptamine release was significantly increased in the brains of mutant mice.

Original languageEnglish
Pages (from-to)1607-1610
Number of pages4
JournalLife Sciences
Volume62
Issue number17-18
DOIs
Publication statusPublished - 1998 Mar 27

Keywords

  • Brain
  • Exploratory behavior
  • Gene targeting
  • Histamine H receptors
  • Locomotor activity
  • Mouse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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