TY - JOUR
T1 - Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry
AU - Pettinella, Caterina
AU - Lee, Seon Hwa
AU - Cipollone, Francesco
AU - Blair, Ian A.
N1 - Funding Information:
We acknowledge the support of NIH grants P50HL70128 and R01CA91016. We also acknowledge the editorial assistance of Dr. Michael Pollack.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - The quantitative analysis of fatty acid composition in atherosclerotic plaques provides a way to monitor the underlying etiology of atherosclerosis. Previously, the method of choice for analyzing fatty acids in biological samples was gas chromatography/mass spectrometry (GC/MS); however, recent developments in electrospray ionization (ESI)/liquid chromatography (LC)/tandem mass spectrometry have made it a superior alternative. Previous research has largely focused on global analyses of intact lipids rather than more targeted analysis of the fatty acids themselves. We have now developed a targeted, stable isotope dilution LC-electrospray ionization/multiple reaction monitoring/MS method for the quantitative analysis of 10 fatty acids (myristic, palmitic, stearic, oleic, linoleic, α-linolenic, γ-linolenic, arachidonic, eicosapentaenoic, and docosahexaenoic acids) using their trimethylaminoethyl ester (TMAE) derivatives to improve sensitivity. The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries.
AB - The quantitative analysis of fatty acid composition in atherosclerotic plaques provides a way to monitor the underlying etiology of atherosclerosis. Previously, the method of choice for analyzing fatty acids in biological samples was gas chromatography/mass spectrometry (GC/MS); however, recent developments in electrospray ionization (ESI)/liquid chromatography (LC)/tandem mass spectrometry have made it a superior alternative. Previous research has largely focused on global analyses of intact lipids rather than more targeted analysis of the fatty acids themselves. We have now developed a targeted, stable isotope dilution LC-electrospray ionization/multiple reaction monitoring/MS method for the quantitative analysis of 10 fatty acids (myristic, palmitic, stearic, oleic, linoleic, α-linolenic, γ-linolenic, arachidonic, eicosapentaenoic, and docosahexaenoic acids) using their trimethylaminoethyl ester (TMAE) derivatives to improve sensitivity. The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries.
KW - Atherosclerosis
KW - Electrospray ionization
KW - Fatty acids
KW - Liquid chromatography/mass spectrometry
KW - Stable isotope dilution
KW - Trimethylaminoethyl ester
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U2 - 10.1016/j.jchromb.2006.11.023
DO - 10.1016/j.jchromb.2006.11.023
M3 - Article
C2 - 17174160
AN - SCOPUS:34247189136
VL - 850
SP - 168
EP - 176
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -