Targeted expression of IP3 sponge and IP3 DsRNA impaires sugar taste sensation in Drosophila

Kazue Usui-Aoki, Ken Matsumoto, Masayuki Koganezawa, Sou Kohatsu, Kunio Isono, Hiroshi Matsubayashi, Masa Toshi Yamamoto, Ryu Ueda, Kuniaki Takahashi, Kaoru Saigo, Katsuhiko Mikoshiba, Daisuke Yamamoto

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the "IP3 sponge," composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.

Original languageEnglish
Pages (from-to)123-141
Number of pages19
JournalJournal of neurogenetics
Volume19
Issue number3-4
DOIs
Publication statusPublished - 2005 Jul

Keywords

  • Behavioral responses
  • Feeding
  • Gal4 transgene
  • IP
  • Inhibitory RNA
  • Sugar sensation

ASJC Scopus subject areas

  • Genetics
  • Cellular and Molecular Neuroscience

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