@article{55f0d19d903245b5b9147845c956a4e0,
title = "Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system",
abstract = "Channelrhodopsin (ChR)-wide receiver (ChRWR), one of the chimeric molecule of ChR1 and ChR2, has several advantages over ChR2 such as improved expression in the plasma membrane and enhanced photocurrent with small desensitization. Here we generated transgenic zebrafish (Danio rerio) expressing ChRWR as a conjugate of EGFP under the regulation of UAS promoter (UAS:ChRWR-EGFP). When crossed with a Gal4 line, SAGFF36B, ChRWR-EGFP was selectively expressed in primary mechanosensory Rohon-Beard (RB) neurons. The direct photoactivation of RB neurons was sufficient to trigger the escape behavior. The UAS:ChRWR-EGFP line could facilitate a variety of investigations of neural networks and behaviors of zebrafish in vivo.",
keywords = "Channelrhodopsin, Escape behavior, Gal4-UAS, Optogenetics, Photostimulation, Rohon-Beard neuron, Somatosensory, Swimming, Transgenic, Zebrafish",
author = "Keiko Umeda and Wataru Shoji and Seiichiro Sakai and Akira Muto and Koichi Kawakami and Toru Ishizuka and Hiromu Yawo",
note = "Funding Information: We are grateful to K. Abe, T. Honjoh, S. Hososhima, T. Igarashi, H. Katow, K. Kikuchi, T. Mishima, T. Okazaki, S. Watanabe and Y. Yokoyama for experimental assistance and to B. Bell for language assistance. This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas “Mesoscopic Neurocircuitry” (No. 23115501 ) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan , Global COE Program (Basic & Translational Research Center for Global Brain Science), MEXT and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO) . Part of this work was carried out under the Cooperative Research Project Program of the Institute of Development, Aging and Cancer, Tohoku University . ",
year = "2013",
month = jan,
doi = "10.1016/j.neures.2012.08.010",
language = "English",
volume = "75",
pages = "69--75",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "1",
}