Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system

Keiko Umeda, Wataru Shoji, Seiichiro Sakai, Akira Muto, Koichi Kawakami, Toru Ishizuka, Hiromu Yawo

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Channelrhodopsin (ChR)-wide receiver (ChRWR), one of the chimeric molecule of ChR1 and ChR2, has several advantages over ChR2 such as improved expression in the plasma membrane and enhanced photocurrent with small desensitization. Here we generated transgenic zebrafish (Danio rerio) expressing ChRWR as a conjugate of EGFP under the regulation of UAS promoter (UAS:ChRWR-EGFP). When crossed with a Gal4 line, SAGFF36B, ChRWR-EGFP was selectively expressed in primary mechanosensory Rohon-Beard (RB) neurons. The direct photoactivation of RB neurons was sufficient to trigger the escape behavior. The UAS:ChRWR-EGFP line could facilitate a variety of investigations of neural networks and behaviors of zebrafish in vivo.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalNeuroscience Research
Volume75
Issue number1
DOIs
Publication statusPublished - 2013 Jan

Keywords

  • Channelrhodopsin
  • Escape behavior
  • Gal4-UAS
  • Optogenetics
  • Photostimulation
  • Rohon-Beard neuron
  • Somatosensory
  • Swimming
  • Transgenic
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

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