Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain

Rie Kusano, Kousuke Fujita, Yasuharu Shinoda, Yuko Nagaura, Hiroshi Kiyonari, Takaya Abe, Toshio Watanabe, Yasuhisa Matsui, Masahiro Fukaya, Hiroyuki Sakagami, Tatsuya Sato, Jun Ichi Funahashi, Motoko Ohnishi, Shinri Tamura, Takayasu Kobayashi

Research output: Contribution to journalLetter

1 Citation (Scopus)

Abstract

PPM1L, a member of the metal-dependent protein phosphatase (PPM) family, is involved in regulating the stress-activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzed ppm1l-deficient mice in order to investigate PPM1L functions in the brain. Our results indicate that ppm1l is highly expressed in the central nervous system during mouse development and that ppm1lΔ/Δ mice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.

Original languageEnglish
Pages (from-to)3606-3615
Number of pages10
JournalFEBS Letters
Volume590
Issue number20
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • MAPK signaling
  • brain development
  • commissural tract
  • ppm1l
  • protein phosphatase
  • striatum

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain'. Together they form a unique fingerprint.

  • Cite this

    Kusano, R., Fujita, K., Shinoda, Y., Nagaura, Y., Kiyonari, H., Abe, T., Watanabe, T., Matsui, Y., Fukaya, M., Sakagami, H., Sato, T., Funahashi, J. I., Ohnishi, M., Tamura, S., & Kobayashi, T. (2016). Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain. FEBS Letters, 590(20), 3606-3615. https://doi.org/10.1002/1873-3468.12429