Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS)

Seon Hwa Lee, Ian A. Blair

Research output: Chapter in Book/Report/Conference proceedingChapter

33 Citations (Scopus)

Abstract

The corona discharge used to generate positive and negative ions under conventional atmospheric pressure chemical ionization (APCI) conditions also provides a source of low-energy gas-phase electrons. This is thought to occur by displacement of electrons from the nitrogen sheath gas. Therefore, suitable analytes can undergo electron capture in the gas phase in a manner similar to that observed for gas chromatography/electron capture negative chemical ionization/mass spectrometry (MS). This technique, which has been named electron-capture APCI (ECAPCI)/MS, mass spectrometry provides an increase in sensitivity of two orders of magnitude when compared with conventional APCI methodology. It is a simple procedure to tag arachidonic acid- and linoleic acid-derived oxidized lipids with an electron-capturing group such as the pentafluorobenzyl (PFB) moiety before analysis. PFB derivatives have previously been used as electron-capturing derivatives because they undergo dissociative electron capture in the gas phase to generate negative ions through the loss of a PFB radical. A similar process occurs under ECAPCI conditions. By monitoring the negative ions that are formed, it is possible to obtain extremely high sensitivity for PFB derivatives of oxidized lipids derived from arachidonic and linoleic acid. A combination of stable isotope dilution methodology and chiral liquid chromatography-ECAPCI/MS makes it possible to resolve and quantify complex mixtures of regioisomeric and enantiomeric oxidized lipids.

Original languageEnglish
Title of host publicationLipidomics and Bioactive Lipids
Subtitle of host publicationSpecialized Analytical Methods and Lipids in Disease
EditorsAlex Brown
Pages159-174
Number of pages16
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume433
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Lee, S. H., & Blair, I. A. (2007). Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS). In A. Brown (Ed.), Lipidomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease (pp. 159-174). (Methods in Enzymology; Vol. 433). https://doi.org/10.1016/S0076-6879(07)33009-7