@article{d11573be87204531a59b1cbe1e1d183e,
title = "Tapetum-specific expression of the Osg6B promoter-β-glucuronidase gene in transgenic rice",
abstract = "The promoter of an anther tapetum-specific gene, Osg6B, was fused to a β-glucuronidase (GUS) gene and introduced into rice by Agrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.",
keywords = "Agrobacterium, Rice, Tapetum-specific promoter, Transformation",
author = "S. Yokoi and T. Tsuchiya and K. Toriyama and K. Hinata",
note = "Funding Information: Acknowledgements The authors wish to thank Dr. Hamid Rashid for the critical review of the manuscript and suggestions for improvement. This study was supported in part by Grants-in-Aid for Special Research on Priority Areas (no. 07281101,07281102 and 07281103; Genetic Dissection of the Sexual Differentiation and Pollination Process in Higher Plants) from the Ministry of Education, Science, Culture and Sports, Japan, and by a grant pioneering research project in biotechnology from the Ministry of Agriculture, Forestry and Fisheries, Japan. S.Y. and T.T. are the recipients of JSPS Fellowships for Japanese Junior Scientists.",
year = "1997",
month = mar,
doi = "10.1007/s002990050240",
language = "English",
volume = "16",
pages = "363--367",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "6",
}