Tandem mass spectrometry analysis of Amadori-glycated phosphatidylethanolamine in human plasma

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Abstract

Amadori-glycated phosphatidylethanolamine (Amadori-PE), a nonenzymatically glycated lipid formed under hyperglycemic conditions, is known as a reliable indicator of lipid glycation in vivo. We have quantified the Amadori-PE concentration in human plasma samples using a reverse-phase liquid chromatography-tandem mass spectrometry with neutral loss scan or multiple reaction monitoring. Amounts of Amadori-PE in plasma of diabetic patients (0.15 mol% of PE), diabetic patients with chronic hemodialysis (0.29 mol% of PE), and nondiabetic patients with chronic hemodialysis (0.13 mol% of PE) are higher than that of the control group (0.08 mol% of PE). In addition, the concentration of Amadori-PE was proportional to that of phosphatidylcholine hydroperoxide, a reliable indicator of membrane lipid peroxidation, in human plasma (P < 0.05). These results indicate that plasma Amadori-PE-glycated lipid product formed under hyperglycemic conditions is an inducer of membrane lipid peroxidation, and therefore lipid glycation plays an active part in the development of human disease.

Original languageEnglish
Pages (from-to)280-283
Number of pages4
JournalAnnals of the New York Academy of Sciences
Volume1043
DOIs
Publication statusPublished - 2005 Jan 1

Keywords

  • Amadori product
  • Glycation
  • Lipid peroxidation
  • Phosphatidylethanolamine
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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