TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development

Yoshiaki Tatsumi, Moe Takeda, Masaru Matsuda, Tohru Suzuki, Hayato Yokoi

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2null, lacking all functional domains, and a hypomorphic mutant, rspo2tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2tsp mutants, but were absent from the rspo2null mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.

    Original languageEnglish
    Pages (from-to)4543-4550
    Number of pages8
    JournalFEBS Letters
    Volume588
    Issue number24
    DOIs
    Publication statusPublished - 2014 Dec 20

    Keywords

    • Fin ray
    • Mutagenesis
    • R-spondin2
    • Skeletogenesis
    • TALEN
    • Zebrafish

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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