Tag-reporter and resin capture - Release strategy in oligosaccharide synthesis

Yukishige Ito; Shino Manabe

doi:10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N

Tag-reporter and resin capture - Release strategy in oligosaccharide synthesis

Yukishige Ito, Shino Manabe

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Realtime reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.

Original language	English
Pages (from-to)	3076-3084
Number of pages	9
Journal	Chemistry - A European Journal
Volume	8
Issue number	14
DOIs	https://doi.org/10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N
Publication status	Published - 2002 Jul 15
Externally published	Yes

Keywords

Capture-release purification
Oligosaccharides
Polymer-supported synthesis
Real-time monitoring
Synthesis design

ASJC Scopus subject areas

Catalysis
Organic Chemistry

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10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N

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AU - Ito, Yukishige

AU - Manabe, Shino

PY - 2002/7/15

Y1 - 2002/7/15

N2 - Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Realtime reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.

AB - Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Realtime reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.

KW - Capture-release purification

KW - Oligosaccharides

KW - Polymer-supported synthesis

KW - Real-time monitoring

KW - Synthesis design

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Tag-reporter and resin capture - Release strategy in oligosaccharide synthesis

Abstract

Keywords

ASJC Scopus subject areas

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