Tachyplesins isolated from hemocytes of southeast asian horseshoe crabs (Carcinoscorpius rotundicauda and Tachypleus gigas): Identification of a new tachyplesin, tachyplesin iii, and a processing intermediate of its precursor

Tatsushi Muta, Tamiji Fujimoto, Hiroshi Nakajima, Sadaaki Iwanaga

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Tachyplesins and their analogs are antimicrobial peptides composed of 17 or 18 amino acid residues present abundantly in acid extracts of hemocyte debris of horseshoe crabs. We purified here tachyplesln isopeptides from hemocytes of two species of Southeast Asian horseshoe crabs, Carcinoscorpius rotundicauda and Tachypleus gigas, and determined t)leir amino acid sequences. The major tachyplesin Isolated from both species was identified, respectively, as tachyplesin I, which had previously been found in hemocytes of the Japanese horseshoe crab (Tachypleus tridentatus). The yield from both species was very high (more than 70 mg per 100 g wet weight of hemocytes), i.e., comparable with that from T. tridentatu.s. In addition to tachyplesin I, a new tachyplesin isopeptide, named tachyplesin III, was also isolated from T. gigas hemocytes, in which an arginine replaced the 15th lysine of tachyplesin I. The carboxyl-terminal residue of the isolated tachyplesins I and III was confirmed, respectively, to be an arginine α-amide by chemical analysis. Furthermore, a tachyplesin peptide derivative with a carboxyl-terminal extension of glycine-lysine was newly found in the hemocytes of C. rotundicauda. It appeared to be an intermediate derived from a tachyplesin precursor during processing to the mature form.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalJournal of biochemistry
Volume108
Issue number2
DOIs
Publication statusPublished - 1990 Aug

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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