TY - JOUR
T1 - Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure
AU - Nakamura, T.
AU - Furunaka, H.
AU - Miyata, T.
AU - Tokunaga, F.
AU - Muta, T.
AU - Iwanaga, S.
AU - Niwa, M.
AU - Takao, T.
AU - Shimonishi, Y.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - A cationic peptide, designated tachyplesin, was isolated from acid extracts of horseshoe crab (Tachypleus tridentatus) hemocyte debris. It consists of 17 residues and the structure determined by Edman degradation is: NH2-K-W-(C-F-R-V-(C-Y-R-G-I-C)-Y-R-R-C-)R-CONH2. The carboxyl-terminal end of this peptide was identified as arginine α-amide and the whole sequence including the α-amide was also confirmed by fast atom bombardment mass spectrometry, indicating a mass value of 2263. Tachyplesin inhibits growth of both Gram-negative and -positive bacteria at low concentrations and formed a complex with bacterial lipopolysaccharide. Tachyplesin seems likely to act as antimicrobial peptide for self-defense in the horseshoe crab against invading microorganisms.
AB - A cationic peptide, designated tachyplesin, was isolated from acid extracts of horseshoe crab (Tachypleus tridentatus) hemocyte debris. It consists of 17 residues and the structure determined by Edman degradation is: NH2-K-W-(C-F-R-V-(C-Y-R-G-I-C)-Y-R-R-C-)R-CONH2. The carboxyl-terminal end of this peptide was identified as arginine α-amide and the whole sequence including the α-amide was also confirmed by fast atom bombardment mass spectrometry, indicating a mass value of 2263. Tachyplesin inhibits growth of both Gram-negative and -positive bacteria at low concentrations and formed a complex with bacterial lipopolysaccharide. Tachyplesin seems likely to act as antimicrobial peptide for self-defense in the horseshoe crab against invading microorganisms.
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M3 - Article
C2 - 3141410
AN - SCOPUS:0023700978
VL - 263
SP - 16709
EP - 16713
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 32
ER -