TY - JOUR
T1 - T-cell variant of classical Hodgkin's lymphoma with nodal and cutaneous manifestations demonstrated by single-cell polymerase chain reaction
AU - Willenbrock, Klaus
AU - Ichinohasama, Ryo
AU - Kadin, Marshall E.
AU - Miura, Ikuo
AU - Terui, Tadashi
AU - Meguro, Kuniaki
AU - Fukuhara, Osamu
AU - DeCoteau, John F.
AU - Hansmann, Martin Leo
N1 - Funding Information:
This study was supported in part by a grant from the Ministry of Health and Welfare, Japan (No. 11-8, 2000) and the Deutsche Krebshilfe, Dr. Mildred Scheel Stiftung. Address reprint requests to: Dr. Klaus Willenbrock, Senckenbergisches Institut für Pathologie, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany. E-mail: Willenbrock@em.uni-frankfurt.de
PY - 2002/9
Y1 - 2002/9
N2 - The atypical cells of CD30+ cutaneous lymphoproliferative disorders (CD30CLD) are commonly of T-cell origin and frequently have a similar morphology as Hodgkin or Reed-Sternberg cells of Hodgkin's lymphoma (HL). HL is one of the tumors associated with CD30CLD. Although most studies support a B-cell derivation of the tumor cells in HL, recently a few cases of classical HL with T-cell genotype have been reported. We report a patient who presented with CD30CLD whose lymph nodes showed classical HL of mixed cellularity subtype at presentation. By single-cell PCR, the same clonal gene rearrangements of the T cell receptor-β gene locus could be assigned to the CD30+ and CD15+ cells of both skin and lymph node. In a lymph node biopsy specimen taken in relapse after several courses of chemotherapy, the CD30+ tumor cells were abundant. The T cell-derived tumor cells displayed aberrant expression of the Pax-5 gene in all specimens. A common clonal origin of both CD30CLD and HL of the lymph node in the patient presented here suggests that HL with T-cell genotype exists in association with CD30CLD as well as in sporadic cases and may share clonal origin with the skin tumor.
AB - The atypical cells of CD30+ cutaneous lymphoproliferative disorders (CD30CLD) are commonly of T-cell origin and frequently have a similar morphology as Hodgkin or Reed-Sternberg cells of Hodgkin's lymphoma (HL). HL is one of the tumors associated with CD30CLD. Although most studies support a B-cell derivation of the tumor cells in HL, recently a few cases of classical HL with T-cell genotype have been reported. We report a patient who presented with CD30CLD whose lymph nodes showed classical HL of mixed cellularity subtype at presentation. By single-cell PCR, the same clonal gene rearrangements of the T cell receptor-β gene locus could be assigned to the CD30+ and CD15+ cells of both skin and lymph node. In a lymph node biopsy specimen taken in relapse after several courses of chemotherapy, the CD30+ tumor cells were abundant. The T cell-derived tumor cells displayed aberrant expression of the Pax-5 gene in all specimens. A common clonal origin of both CD30CLD and HL of the lymph node in the patient presented here suggests that HL with T-cell genotype exists in association with CD30CLD as well as in sporadic cases and may share clonal origin with the skin tumor.
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U2 - 10.1097/01.LAB.0000027839.98023.5A
DO - 10.1097/01.LAB.0000027839.98023.5A
M3 - Article
C2 - 12218070
AN - SCOPUS:0036718617
VL - 82
SP - 1103
EP - 1109
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 9
ER -