T cell receptor repertoire in BALB/c mice varies according to tissue type, sex, age, and hydrocortisone treatment

Kazutaka Kitaura, Kiichi Kanayama, Yoshiki Fujii, Noriyuki Shiobara, Konagi Tanaka, Ichiro Kurane, Satsuki Suzuki, Tsunetoshi Itoh, Ryuji Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Diversity in T cell recognition of antigens is determined by diverse usage of T cell receptor (TCR) repertoire. TCR repertoire analysis provides fundamental information for understanding T cell immune responses in the pathogenesis of various diseases. In the present study, we examined the TCR repertoire in various tissues in normal BALB/c mice. The TCR α chain variable region repertoires were consistent among the spleen, lymph nodes, and the thymus. The TCR β chain variable region (TCRBV) repertoires were consistent between the spleen and lymph nodes, but different in the thymus. The TCR repertoires also differed in the lungs and the intestinal tract. The TCR repertoires were consistent between male and female mice, except for TCRBV15-1. TCR repertoire was almost similar in 3- and 7-week-old mice, except for TCRBV1-1, 8-3, and 14-1. The present findings suggest that the TCR repertoire of mice varies according to tissue type, sex and age. Additional analysis of the TCR repertoire, i.e., the effect of hydrocortisone (HC), was carried out. After the HC treatment, although the thymic T cells decreased to one-tenth, only a small fraction of CD4+CD8+ T cells survived the treatment. Furthermore, the percentages of thymic T cells bearing TCRBV3-1, 5-1, 5-2, and 16-1 substantially decreased, but the percentage of cells bearing TCRBV12-1 did not decrease. The present findings suggest that the HC susceptibility of immature thymic T cells is different between TCR families.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalExperimental Animals
Volume58
Issue number2
DOIs
Publication statusPublished - 2009 Sep 22

Keywords

  • FACS
  • Hydrocortisone
  • Real-time PCR
  • Repertoire
  • T cell receptor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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    Kitaura, K., Kanayama, K., Fujii, Y., Shiobara, N., Tanaka, K., Kurane, I., Suzuki, S., Itoh, T., & Suzuki, R. (2009). T cell receptor repertoire in BALB/c mice varies according to tissue type, sex, age, and hydrocortisone treatment. Experimental Animals, 58(2), 159-168. https://doi.org/10.1538/expanim.58.159