Systemic distribution and tissue localizations of human 17beta-hydroxysteroid dehydrogenase type 12

Nobuyuki Sakurai, Yasuhiro Miki, Takashi Suzuki, Keiko Watanabe, Takashi Narita, Kozue Ando, Tetsu M.C. Yung, Daisuke Aoki, Hironobu Sasano, Hiroshi Handa

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The 17β-hydroxysteroid dehydrogenases (HSDs) are enzymes that catalyze the reduction of 17-ketosteroids or the oxidation of 17β-hydroxysteroids. 17β-HSD type 12, the most recently cloned member of this gene family, was classified into the 17β-HSD family based on sequence homology, rather than steroid catalyzing activity. Meanwhile, it has been reported that 17β-HSD type 12 may be involved in fatty acid synthesis. To better understand the role of 17β-HSD type 12 in lipid metabolism, we determined the detailed systemic distribution and tissue localizations of 17β-HSD type 12, which, due partly to the lack of antibodies, had not yet been studied. We carried out these investigations by quantitative reverse transcription (RT)-PCR, Northern blot analysis, and immunohistochemistry, using an antibody against 17β-HSD type 12 that we have generated. 17β-HSD type 12 is highly expressed in organs related to lipid metabolism such as liver, kidney, heart and skeletal muscle. 17β-HSD type 12 is also detected in endocrine-related organs such as pancreas, pituitary gland, adrenal gland, testis and placenta, and in the gastrointestinal tract, which point to the possible involvement of 17β-HSD type 12 in the regulation of lipid biosynthesis and steroid metabolism. These results support previous reports and solidify the possibility that 17β-HSD type 12 may play critical roles in the physiological processes, such as fatty acid synthesis, in addition to the steroid metabolism.

Original languageEnglish
Pages (from-to)174-181
Number of pages8
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume99
Issue number4-5
DOIs
Publication statusPublished - 2006 Jun 1

Keywords

  • 17Beta-hydroxysteroid dehydrogenase type 12
  • Fatty acid
  • Immunohistochemistry
  • Quantitative RT-PCR

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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