Syntrophomonas palmitatica sp. nov., an anaerobic syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge

Masashi Hatamoto, Hiroyuki Imachi, Sanae Fukayo, Akiyoshi Ohashi, Hideki Harada

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

A mesophilic, syntrophic, fatty-acid-oxidizing anaerobic strain, designated MPAT, was isolated from granular sludge in a mesophilic upflow anaerobic sludge blanket reactor used to treat palm oil mill effluent. Cells were slightly curved, non-motile rods. Spore formation was not observed. The optimal temperature for growth was around 37 °C and optimal pH for growth was 7.0. Strain MPAT was able to grow on crotonate or pentenoate plus butyrate in pure culture. In co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei, strain MPAT was able to oxidize straight-chain saturated fatty acids with carbon chain lengths of C4-C18. The strain was unable to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, iron(III) or DIVISO as an electron acceptor. The G+C content of the DNA was 45.0 mol%. Based on comparative 1 6S rRNA gene sequence analysis, strain MPAT was found to be a member of the genus Syntrophomonas and was most closely related to the type strains of Syntrophomonas curvata and Syntrophomonas sapovorans (sequence similarities of 94%). Genetic and phenotypic characteristics demonstrated that strain MPAT represents a novel species, for which the name Syntrophomonas palmitatica sp. nov. is proposed. The type strain is MPAT (=JCM 14374T=NBRC 102128T=DSM 18709T.

Original languageEnglish
Pages (from-to)2137-2142
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume57
Issue number9
DOIs
Publication statusPublished - 2007 Sep

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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