Synthesis of Staudinger-type molecular probe for catch-and-release purification of the binding protein for potassium isolespedezate, a leaf-closing substance of leguminous plant

Tomohiko Fujii, Nobuki Kato, Izumi Iwakura, Yoshiyuki Manabe, Minoru Ueda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We synthesized azide-containing photoaffinity probe 1 based on the structure of potassium isolespedezate. This probe can be used for catch-and-release-mechanism purification of binding protein for 1: photo-crosslinking with 1 gave azide-labeled receptor which can be captured by phosphane-linked gel matrix by the Staudinger ligation. After washing the gel, the caught binding protein can be released by the reductive cleavage of disulfide bond in 1. This process can be used as a convenient method for the purification of binding protein for bioactive natural product.

Original languageEnglish
Pages (from-to)52-53
Number of pages2
JournalChemistry Letters
Volume37
Issue number1
DOIs
Publication statusPublished - 2008 Jan 5

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Synthesis of Staudinger-type molecular probe for catch-and-release purification of the binding protein for potassium isolespedezate, a leaf-closing substance of leguminous plant'. Together they form a unique fingerprint.

  • Cite this