Synthesis of SiO2-coated magnetite nanopartiçles and immobilization of proteins on them

Yoshio Kobayashi, Mayumi Yoshida, Daisuke Nagao, Yasuo Ando, Terunobu Miyazaki, Mikio Konno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

This paper describes a method for silica-coating of magnetite nanoparticles and immobilization of proteins on the silica-coated particles. The magnetite nanoparticles were prepared according to the Massart method. The silica-coating was performed in aqueous/ethanolic solution with tetraethoxyorthosilicate and ammonia aqueous solution in the presence of the magnetite nanoparticles. TEM observation revealed that the nanoparticles were coated with silica shell. Saturation magnetization of the SiO2-coated particles was 52.4 emu/gFe3O4, which indicated that the magnetite nanoparticles were chemically undamaged during the silica-coating. The protein immobilization was performed in aqueous solution with bovine serum albumin in the presence of the silica-coated particles surface-modified with 3-aminopropyltrimethoxysilane and glutaraldehyde. UV-VIS absorption spectroscopy revealed that the BSA was adsorbed on the surface-modified particles.

Original languageEnglish
Title of host publicationProceedings of the 2nd Int. Conf. Characterization and Control of Interfaces for High Quality Advanced Materials, and Joining Technology for New Metallic Glasses and Inorganic Materials
Pages135-141
Number of pages7
Publication statusPublished - 2007
Event2nd International Conference on Characterization and Control of Interfaces for High Quality Advanced Materials, and Joining Technology for New Metallic Glasses and Inorganic Materials - Kurashiki, Japan
Duration: 2006 Sep 62006 Sep 9

Publication series

NameCeramic Transactions
Volume198
ISSN (Print)1042-1122

Other

Other2nd International Conference on Characterization and Control of Interfaces for High Quality Advanced Materials, and Joining Technology for New Metallic Glasses and Inorganic Materials
Country/TerritoryJapan
CityKurashiki
Period06/9/606/9/9

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

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