Synthesis of silica-coated agi nanoparticles and immobilization of proteins on them

Yoshio Kobayashi, Marie Minato, Kazuaki Ihara, Masahide Sato, Noboru Suzuki, Motohiro Takeda, Noriaki Ohuchi, Atsuo Kasuya

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This paper describes a method for preparing silica-coated silver iodide (Agl/Si0 2) particles and immobilizing proteins on the Agl/Si0 2 particles. Colloid solution of AgI particles was prepared by mixing AgCIO 4 aqueous solution and KI aqueous solution. Silica-coating of the AgI particles was performed by adding 3-mercaptopropyltrimethoxysilane, tetraethylorthosilicate/ethanol solution and NaOH aqueous solution successively to the AgI colloid solution. TEM observation revealed that the AgI nanoparticles were coated with uniform silica shell. The Agl/Si0 2 particles were surface-modified with 3-aminopropyltrimethoxysilane and succinic anhydride. It was confirmed by XPS measurement that amino group or carboxyl group was introduced onto the Agl/Si0 2 particles. Protein-immobilization was performed in aqueous solution with bovine serum albumin (BSA) in the presence of the surface-modified Agl/Si0 2 particles. UV-VlS absorption spectroscopy revealed that the BSA was adsorbed on the surface-modified particles.

Original languageEnglish
Pages (from-to)7758-7761
Number of pages4
JournalJournal of Nanoscience and Nanotechnology
Volume10
Issue number11
DOIs
Publication statusPublished - 2010 Nov

Keywords

  • AgI
  • Core-shell
  • Nanoparticle
  • Protein immobilization
  • Silica-coating
  • Sol-gel

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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