Synthesis of RNA Crosslinking Oligonucleotides Modified with 2-Amino-7-Deaza-7-Propynyl-6-Vinylpurine

Ken Yamada, Yusuke Abe, Fumi Nagatsugi

Research output: Contribution to journalArticlepeer-review

Abstract

This article describes procedures to synthesize 2′-OMe-RNA modified with cross-linkable 2-amino-7-deaza-7-propynyl-6-vinylpurine (ADpVP) and preparation of the RNA-crosslinking experiment in vitro. All synthesis steps yield the desired compound in moderate or high yield without expensive chemical reagents or specific devices. The crosslink-active form of modified RNA can also be purified by commonly used reversed-phase HPLC, can be stored at −80°C after lyophilization for a few days, and is ready to use for crosslinking experiments. This crosslink-active RNA can efficiently form covalent bonds with complementary RNA in a sequence-specific manner.

Original languageEnglish
Article numbere79
JournalCurrent Protocols in Nucleic Acid Chemistry
Volume77
Issue number1
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • 7-deaza-guanosine
  • RNA crosslink
  • nucleoside
  • oligonucleotide

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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