Abstract
This article describes procedures to synthesize 2′-OMe-RNA modified with cross-linkable 2-amino-7-deaza-7-propynyl-6-vinylpurine (ADpVP) and preparation of the RNA-crosslinking experiment in vitro. All synthesis steps yield the desired compound in moderate or high yield without expensive chemical reagents or specific devices. The crosslink-active form of modified RNA can also be purified by commonly used reversed-phase HPLC, can be stored at −80°C after lyophilization for a few days, and is ready to use for crosslinking experiments. This crosslink-active RNA can efficiently form covalent bonds with complementary RNA in a sequence-specific manner.
Original language | English |
---|---|
Article number | e79 |
Journal | Current Protocols in Nucleic Acid Chemistry |
Volume | 77 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2019 Jun |
Keywords
- 7-deaza-guanosine
- RNA crosslink
- nucleoside
- oligonucleotide
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry