Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

Yoichiro Hoshina, Yoshifumi Yamada, Hiroshi Tanaka, Takayuki Doi, Takashi Takahashi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The design and solid-phase synthesis of effective fluorescent-labeled aeruginosin derivatives and their application to the fluorescence correlation spectroscopy (FCS)-based competitive binding assay of an aeruginosin library are described. The phenolic hydroxyl group on the (R)-3-(4-hydroxyphenyl)lactic acid (d-Hpla) residue was observed to be suitable for connecting Rhodamine green derivative with minimum loss of biological activity. In addition, the FCS-based binding assay of the library using fluorescent-labeled chemical probes was also achieved.

Original languageEnglish
Pages (from-to)2904-2907
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume17
Issue number10
DOIs
Publication statusPublished - 2007 May 15
Externally publishedYes

Keywords

  • Chemical biology
  • Combinatorial chemistry
  • Fluorescence correlation spectroscopy
  • Solid-phase synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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