TY - JOUR
T1 - Synthesis of an allergy inducing tetrasaccharide “4P-X”
AU - Moriya, Takashi
AU - Nagahata, Naoki
AU - Odaka, Rei
AU - Nakamura, Hirohide
AU - Yoshikawa, Jun
AU - Kurashima, Katsumi
AU - Saito, Tadao
PY - 2017/1/1
Y1 - 2017/1/1
N2 - 4P-X (β-D-galactopyranosyl-(1 → 4)-β-D-galactopyranosyl-(1 → 6)-[β-D-galactopyranosyl-(1 → 4)]-β-D-glucopyranose) is included in galacto-oligosaccharides (GOSs) produced by β-galactosidase derived from Bacillus circulans. 4P-X has been known to induce particularly strong allergies. High purity 4P-X is essential for use as a standard to quantify the amount of 4P-X in GOSs; however, the isolation of high purity 4P-X has never been reported. In this study, we achieved the synthesis of 4P-X by a combination of organic and enzymatic chemical syntheses in a short time. This is the first report of isolated, high purity 4P-X.
AB - 4P-X (β-D-galactopyranosyl-(1 → 4)-β-D-galactopyranosyl-(1 → 6)-[β-D-galactopyranosyl-(1 → 4)]-β-D-glucopyranose) is included in galacto-oligosaccharides (GOSs) produced by β-galactosidase derived from Bacillus circulans. 4P-X has been known to induce particularly strong allergies. High purity 4P-X is essential for use as a standard to quantify the amount of 4P-X in GOSs; however, the isolation of high purity 4P-X has never been reported. In this study, we achieved the synthesis of 4P-X by a combination of organic and enzymatic chemical syntheses in a short time. This is the first report of isolated, high purity 4P-X.
KW - 4P-X
KW - Allergy
KW - Galacto-oligosaccharides
KW - Oligosaccharide synthesis
UR - http://www.scopus.com/inward/record.url?scp=85009517884&partnerID=8YFLogxK
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U2 - 10.1016/j.carres.2016.11.013
DO - 10.1016/j.carres.2016.11.013
M3 - Article
C2 - 28107656
AN - SCOPUS:85009517884
VL - 439
SP - 44
EP - 49
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
ER -