TY - JOUR
T1 - Synthesis and surface functionalization of Fe3O4-SiO2 core-shell nanoparticles with 3-glycidoxypropyltrimethoxysilane and 1,1′-carbonyldiimidazole for bio-applications
AU - Ta, Thi Kieu Hanh
AU - Trinh, Minh Thuong
AU - Long, Nguyen Viet
AU - Nguyen, Thi Thanh My
AU - Nguyen, Thi Lien Thuong
AU - Thuoc, Tran Linh
AU - Phan, Bach Thang
AU - Mott, Derrick
AU - Maenosono, Shinya
AU - Tran-Van, Hieu
AU - Le, Van Hieu
PY - 2016/1/1
Y1 - 2016/1/1
N2 - In our research, we have presented the controlled synthesis of Fe3O4 nanoparticles (NPs) with a size of about 10 nm coated with SiO2 shells for bio-applications. On this basis, the controlled synthesis of Fe3O4-SiO2 core-shell nanoparticles and their surface functionalization with 3-glycidoxypropyltrimethoxysilane (GPS) and 1,1′-carbonyldiimidazole (CDI) has been presented with a facile synthetic process. The as-prepared Fe3O4-SiO2-GPS-CDI core-multishell NPs can bind proteins. Therefore, recombinant protein A/G (pA/G) was efficiently coupled onto the surface of NPs via CDI groups, creating a complete coverage. Antibodies (αT IgG) were also conjugated on Fe3O4-SiO2-GPS-CDI-pA/G, i.e. 9 mg αT antibodies per 1 g NPs. After the surface functionalization of the magnetic nanoparticles, their superparamagnetism was reduced by a factor of about threefold in Fe3O4-SiO2, and fivefold in Fe3O4-SiO2-GPS-CDI-pA/G in comparison with that of the naked Fe3O4 NPs. The NPs conjugated with αT IgG could bind and remove 1 × 105 cells per 0.25 mg NPs in vitro. Finally, the new models of surface functionalization of magnetic nanoparticles have been proposed for promising bioconjugations in our further research.
AB - In our research, we have presented the controlled synthesis of Fe3O4 nanoparticles (NPs) with a size of about 10 nm coated with SiO2 shells for bio-applications. On this basis, the controlled synthesis of Fe3O4-SiO2 core-shell nanoparticles and their surface functionalization with 3-glycidoxypropyltrimethoxysilane (GPS) and 1,1′-carbonyldiimidazole (CDI) has been presented with a facile synthetic process. The as-prepared Fe3O4-SiO2-GPS-CDI core-multishell NPs can bind proteins. Therefore, recombinant protein A/G (pA/G) was efficiently coupled onto the surface of NPs via CDI groups, creating a complete coverage. Antibodies (αT IgG) were also conjugated on Fe3O4-SiO2-GPS-CDI-pA/G, i.e. 9 mg αT antibodies per 1 g NPs. After the surface functionalization of the magnetic nanoparticles, their superparamagnetism was reduced by a factor of about threefold in Fe3O4-SiO2, and fivefold in Fe3O4-SiO2-GPS-CDI-pA/G in comparison with that of the naked Fe3O4 NPs. The NPs conjugated with αT IgG could bind and remove 1 × 105 cells per 0.25 mg NPs in vitro. Finally, the new models of surface functionalization of magnetic nanoparticles have been proposed for promising bioconjugations in our further research.
KW - 1,1′-Carbonyldiimidazole
KW - 3-Glycidoxypropyltrimethoxysilane
KW - Core-shell FeO-SiO nanoparticles
KW - FeO-SiO-GPS-CDI-pA/G-αT
KW - Protein BSA
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U2 - 10.1016/j.colsurfa.2016.05.008
DO - 10.1016/j.colsurfa.2016.05.008
M3 - Article
AN - SCOPUS:84979879982
VL - 504
SP - 376
EP - 383
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
SN - 0927-7757
ER -