Synthesis and surface functionalization of Fe3O4-SiO2 core-shell nanoparticles with 3-glycidoxypropyltrimethoxysilane and 1,1′-carbonyldiimidazole for bio-applications

Thi Kieu Hanh Ta, Minh Thuong Trinh, Nguyen Viet Long, Thi Thanh My Nguyen, Thi Lien Thuong Nguyen, Tran Linh Thuoc, Bach Thang Phan, Derrick Mott, Shinya Maenosono, Hieu Tran-Van, Van Hieu Le

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


In our research, we have presented the controlled synthesis of Fe3O4 nanoparticles (NPs) with a size of about 10 nm coated with SiO2 shells for bio-applications. On this basis, the controlled synthesis of Fe3O4-SiO2 core-shell nanoparticles and their surface functionalization with 3-glycidoxypropyltrimethoxysilane (GPS) and 1,1′-carbonyldiimidazole (CDI) has been presented with a facile synthetic process. The as-prepared Fe3O4-SiO2-GPS-CDI core-multishell NPs can bind proteins. Therefore, recombinant protein A/G (pA/G) was efficiently coupled onto the surface of NPs via CDI groups, creating a complete coverage. Antibodies (αT IgG) were also conjugated on Fe3O4-SiO2-GPS-CDI-pA/G, i.e. 9 mg αT antibodies per 1 g NPs. After the surface functionalization of the magnetic nanoparticles, their superparamagnetism was reduced by a factor of about threefold in Fe3O4-SiO2, and fivefold in Fe3O4-SiO2-GPS-CDI-pA/G in comparison with that of the naked Fe3O4 NPs. The NPs conjugated with αT IgG could bind and remove 1 × 105 cells per 0.25 mg NPs in vitro. Finally, the new models of surface functionalization of magnetic nanoparticles have been proposed for promising bioconjugations in our further research.

Original languageEnglish
Pages (from-to)376-383
Number of pages8
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Publication statusPublished - 2016
Externally publishedYes


  • 1,1′-Carbonyldiimidazole
  • 3-Glycidoxypropyltrimethoxysilane
  • Core-shell FeO-SiO nanoparticles
  • FeO-SiO-GPS-CDI-pA/G-αT
  • Protein BSA

ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


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