Synthesis and Properties of Hyaluronic Acid Conjugated Nucleic Acid Analogs—1: Synthesis of Deacetylhyaluronan and Introduction of Nucleic Acid Bases

Takehiko Wada, Suwabun Chirachanchai, Naoto Izawa, Yoshiaki Inaki, Kchi Takemoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The conjugation of nucleic acid base with hyaluronan was achieved by using the activated ester of pentachlorophenyl trichloroacetate. The conditions of de-N-acetylation of sodium hyaluronic acid were studied. In low concentrations of NaOH, the degree of deacetylation was 26%, while in 7.4N NaOH, the degree of deacetylation was 76% and the viscosity was 1.12 dL/g. Thymine and 5-fluorouracil bases were quantitatively conjugated to deacetylhyaluronan in 65% and 51%, respectively. The interaction of thymine hyaluronan conjugate with the complementary base of polyadenylate showed a small hypochromicity.

Original languageEnglish
Pages (from-to)429-447
Number of pages19
JournalJournal of Bioactive and Compatible Polymers
Volume9
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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