Synthesis and function of maltopentaose-substituted poly(L-lysine)

Mio Yamazaki; Shin Ichi Sawada; Nobuyuki Morimoto; Kazunari Akiyoshi

Synthesis and function of maltopentaose-substituted poly(L-lysine)

Mio Yamazaki, Shin Ichi Sawada, Nobuyuki Morimoto, Kazunari Akiyoshi

Research output: Contribution to conference › Paper › peer-review

Abstract

Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Externally published	Yes
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Amylose
DNA
Enzymatic phosphorylation
Poly-L-lysine

ASJC Scopus subject areas

Engineering(all)

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title = "Synthesis and function of maltopentaose-substituted poly(L-lysine)",

abstract = "Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.",

keywords = "Amylose, DNA, Enzymatic phosphorylation, Poly-L-lysine",

author = "Mio Yamazaki and Sawada, {Shin Ichi} and Nobuyuki Morimoto and Kazunari Akiyoshi",

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T1 - Synthesis and function of maltopentaose-substituted poly(L-lysine)

AU - Yamazaki, Mio

AU - Sawada, Shin Ichi

AU - Morimoto, Nobuyuki

AU - Akiyoshi, Kazunari

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.

AB - Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.

KW - Amylose

KW - DNA

KW - Enzymatic phosphorylation

KW - Poly-L-lysine

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