Synthesis and function of maltopentaose-substituted poly(L-lysine)

Mio Yamazaki, Shin Ichi Sawada, Nobuyuki Morimoto, Kazunari Akiyoshi

Research output: Contribution to conferencePaperpeer-review

Abstract

Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Amylose
  • DNA
  • Enzymatic phosphorylation
  • Poly-L-lysine

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Synthesis and function of maltopentaose-substituted poly(L-lysine)'. Together they form a unique fingerprint.

Cite this