Abstract
Amylose is a unique biodegradable polysaccharide having low toxicity and immunogenicity. It can be enzymatically polymerized from a primer, maltopentaose (MP) by phosphorylase. In this study, a novel amyloseprimer, poly (L-lysine) grafted maltopentaose (MP-g-PLL) was designed. PLL is a cationic polymer that can interact with DNA. MP-PLLs with 1%, 8% and 15% of MP by mole ratio were synthesized by reductive animation reaction. The MP-PLL copolymers were then enzymatically polymerized in the presence of phosphorylase and α-D-glucose 1-phosphate. The degree of polymerization (DP) was linearly increased in accordance to the polymerization time. The rate of polymerization of MP-PLLs has been enhanced compared to the unmodified MP. The DP of MP-PLLs can be controlled by phosphorylase concentration and polymerization time.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - 2005 Dec 1 |
| Externally published | Yes |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country/Territory | Japan |
| City | Yokohama |
| Period | 05/5/25 → 05/5/27 |
Keywords
- Amylose
- DNA
- Enzymatic phosphorylation
- Poly-L-lysine
ASJC Scopus subject areas
- Engineering(all)