Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

Fumi Nagatsugi, Ryusuke Nakahara, Kaori Inoue, Shigeki Sasaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalArchiv der Pharmazie
Volume341
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1

Keywords

  • Conjugate
  • Detection
  • Luciferase
  • Nucleic acid
  • Oligodeoxynucleotide

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

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