Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells

Kenzo Ohsawa; Yumiko Hayakawa; Mikio Nishizawa; Takashi Yamagami; Hiroshi Yamamoto; Noboru Yanaihara; Hiroshi Okamoto

doi:10.1016/0006-291X(85)91890-X

Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells

Kenzo Ohsawa, Yumiko Hayakawa, Mikio Nishizawa, Takashi Yamagami, Hiroshi Yamamoto, Noboru Yanaihara, Hiroshi Okamoto

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Research output: Contribution to journal › Article › peer-review

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Abstract

The effects of dibutyryl cAMP (Bt₂cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt₂cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt₂cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt₂cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.

Original language	English
Pages (from-to)	885-891
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	132
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91890-X
Publication status	Published - 1985 Nov 15

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells. / Ohsawa, Kenzo; Hayakawa, Yumiko; Nishizawa, Mikio; Yamagami, Takashi; Yamamoto, Hiroshi; Yanaihara, Noboru; Okamoto, Hiroshi.

In: Biochemical and biophysical research communications, Vol. 132, No. 3, 15.11.1985, p. 885-891.

Research output: Contribution to journal › Article › peer-review

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title = "Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells",

abstract = "The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.",

author = "Kenzo Ohsawa and Yumiko Hayakawa and Mikio Nishizawa and Takashi Yamagami and Hiroshi Yamamoto and Noboru Yanaihara and Hiroshi Okamoto",

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AU - Ohsawa, Kenzo

AU - Hayakawa, Yumiko

AU - Nishizawa, Mikio

AU - Yamagami, Takashi

AU - Yamamoto, Hiroshi

AU - Yanaihara, Noboru

AU - Okamoto, Hiroshi

PY - 1985/11/15

Y1 - 1985/11/15

N2 - The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.

AB - The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.

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Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells

Abstract

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