TY - JOUR
T1 - Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells
AU - Ohsawa, Kenzo
AU - Hayakawa, Yumiko
AU - Nishizawa, Mikio
AU - Yamagami, Takashi
AU - Yamamoto, Hiroshi
AU - Yanaihara, Noboru
AU - Okamoto, Hiroshi
N1 - Funding Information:
ACKNOWLEDGEMENTS: This work was supported in part by Grants-in-Aid for Scientific Research and for Cancer Research from the Ministry of Education, Science and Culture, Japan.
PY - 1985/11/15
Y1 - 1985/11/15
N2 - The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.
AB - The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.
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U2 - 10.1016/0006-291X(85)91890-X
DO - 10.1016/0006-291X(85)91890-X
M3 - Article
AN - SCOPUS:0022398879
VL - 132
SP - 885
EP - 891
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -