Synergistic stimulation of VIP PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells

Kenzo Ohsawa, Yumiko Hayakawa, Mikio Nishizawa, Takashi Yamagami, Hiroshi Yamamoto, Noboru Yanaihara, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP PHM-27 gene expression through independent pathways.

Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalBiochemical and biophysical research communications
Volume132
Issue number3
DOIs
Publication statusPublished - 1985 Nov 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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