Synergistic stimulation of histamine release from rat peritoneal mast cells by 12-O-tetradecanoylphorbol 13-acetate (TPA)-type and non-TPA-type tumor promoters

Kazuo Ohuchi, Noriyasu Hirasawa, Chikako Takahashi, Masako Watanabe, Susumu Tsurufuji, Hirota Fujiki, Masami Suganuma, Hiromi Hakii, Takashi Sugimura, S. Brøgger Christensen

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Thapsigargin, a non-TPA (12-O-tetradecanoylphorbol 13-acetate)-type tumor promoter, provoked histamine release from rat peritoneal mast cells at concentrations above 30 ng/ml, but not at 10 ng/ml. TPA-type tumor promoters such as TPA, teleocidin and aplysiatoxin released very little, if any, histamine even at 100 ng/ml. When mast cells were incubated in medium containing thapsigargin at 10 ng/ml and varying concentrations of TPA-type tumor promoters, histamine release was increased synergistically. Maximum synergistic effects were observed at 10 ng/ml of each TPA-type tumor promoter. Palytoxin, another non-TPA-type tumor promoter, having no effect on histamine release at up to 10 pg/ml, also induced histamine release in the presence of 10 ng/ml of each TPA-type tumor promoter. However, no synergistic effect on histamine release was observed when mast cells were incubated in medium containing two different non-TPA-type tumor promoters, e.g., 10 ng/ml thapsigargin and 10 pg/ml palytoxin, or in medium containing two different TPA-type tumor promoters, e.g., TPA and teleocidin, TPA and aplysiatoxin, or teleocidin and aplysiatoxin (all at 10 ng/ml). These results suggest that the release of histamine from mast cells is stimulated synergistically under the mutual influence of TPA-type tumor promoters and non-TPA-type tumor promoters.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalBBA - Molecular Cell Research
Volume887
Issue number1
DOIs
Publication statusPublished - 1986 Jun 16

Keywords

  • (Rat mast cell)
  • Aplysiatoxin
  • Histamine
  • Palytoxin
  • Phorbol ester
  • Teleocidin
  • Thapsigargin
  • Tumor promoter

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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