Synergistic effect of thermal energy on bactericidal action of photolysis of H 2O 2in relation to acceleration of hydroxyl radical generation

Midori Shirato, Hiroyo Ikai, Keisuke Nakamura, Eisei Hayashi, Taro Kanno, Keiichi Sasaki, Masahiro Kohno, Yoshimi Niwano

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29 Citations (Scopus)

Abstract

The purpose of the present study is to evaluate the effect of thermal energy on the yield of and the bactericidal action of hydroxyl radical generated by photolysis of H 2O 2. Different concentrations of H 2O 2 (250, 500, 750, and 1,000 mM) were irradiated with light-emitting diodes (LEDs) at a wavelength of 400 ± 20 nm at 25°C to generate hydroxyl radical. The 500 mM H 2O 2 was irradiated with the LEDs at different temperatures (25, 35, 45, and 55°C). Electron spin resonance spin trapping analysis showed that the yield of hydroxyl radicals increased with the temperature, as well as the concentration of H 2O 2. Streptococcus mutans and Enterococcus faecalis were used in the bactericidal assay. The LED-light irradiation of the bacterial suspensions in 500 mM H 2O 2at 25°C could hardly kill the bacteria within 3 min, while the bactericidal effect was markedly enhanced with the temperature rise. For instance, a temperature increase to 55°C resulted in >99.999% reduction of viable counts of both bacterial species only within 1 min. The photolysis of 500 mM H 2O 2 at 55°C could reduce the viable counts of bacteria more efficiently than did the photolysis of 1,000 mM H 2O 2 at 25°C, although the yields of hydroxyl radical were almost the same under the both conditions. These findings suggest that the thermal energy accelerates the generation of hydroxyl radical by photolysis of H 2O 2, which in turn results in a synergistic bactericidal effect of hydroxyl radical and thermal energy.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalAntimicrobial agents and chemotherapy
Volume56
Issue number1
DOIs
Publication statusPublished - 2012 Jan

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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