Synchronized and timing-stabilized pulse generation from a gain-switched laser diode for stimulated Raman scattering microscopy

Kyoya Tokunaga, Yi Cheng Fang, Hiroyuki Yokoyama, Yasuyuki Ozeki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a picosecond laser source based on a gain-switched laser diode (GS-LD) that can be applied to stimulated Raman scattering (SRS) microscopy. A 1.06-μm GS-LD was used to generate 14-ps pulses at a repetition rate of 38 MHz. The GS-LD was driven by 200-ps electrical pulses, which were triggered through a toggle flip-flop (T-FF). As a result, the GS-LD pulses were subharmonically synchronized to Ti:sapphire laser (TSL) pulses at a repetition rate of 76 MHz. We investigated the timing jitter of GS-LD pulses and found it to be less than 2.5 ps. We also show that the trigger delay can be less sensitive to the optical power of TSL pulses by controlling the threshold voltage of the T-FF. As a result, GS-LD pulses sufficiently overlapped with TSL pulses even when we scanned the wavelength of the TSL pulses. We demonstrate the SRS imaging of HeLa cells with GS-LD pulses and TSL pulses, proving that GS-LD is readily applicable to SRS microscopy as a compact and stable pulse source.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVI
EditorsPeter T. C. So, Karsten Konig, Ammasi Periasamy, Karsten Konig
PublisherSPIE
ISBN (Electronic)9781628419467
DOIs
Publication statusPublished - 2016 Jan 1
EventMultiphoton Microscopy in the Biomedical Sciences XVI - San Francisco, United States
Duration: 2016 Feb 142016 Feb 16

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9712
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XVI
CountryUnited States
CitySan Francisco
Period16/2/1416/2/16

Keywords

  • Raman microscopy
  • gain-switched laser diode
  • semiconductor laser
  • stimulated Raman scattering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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