Synaptotagmin-like protein 1-3: A novel family of c-terminal-type tandem c2 proteins

Mitsunori Fukuda, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Synaptotagmins (Syt), rabphilin-3A, and Doc2 belong to a family of carboxyl terminal type (C-type) tandem C2 proteins and are thought to be involved in vesicular trafficking. We have cloned and characterized a novel family of C-type tandem C2 proteins, designated Slp1-3 (synaptotagmin-like protein 1-3). The Slp1-3 C2 domains show high homology to granuphilin-a C2 domains, but the amino-terminal domain of Slp1-3 does not contain any known protein motifs or a transmembrane domain. A subcellular fractionation study indicated that Slp1-3 proteins are peripheral membrane proteins. Phospholipid binding experiments indicated that Slp3 is a Ca2+-dependent isoform, but Slp1 and Slp2 are Ca2+-independent isoforms, because only the Slp3 C2A domain showed Ca2+-dependent phospholipid binding activity. The C-terminus of Slp1-3 also bound neurexin Iα in vitro, in the same manner as Syt family proteins, which may be important for the membrane association of Slp1-3. In addition, Sip family proteins are differentially distributed in different mouse tissues and at different developmental stages.

Original languageEnglish
Pages (from-to)1226-1233
Number of pages8
JournalBiochemical and biophysical research communications
Volume281
Issue number5
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Keywords

  • C2 domain
  • Doc2
  • Granuphilin
  • Phospholipid binding
  • Rabphilin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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