@article{9ecad89e6c204166a6bbb77a08e7a34c,
title = "Synaptic pruning through glial synapse engulfment upon motor learning",
abstract = "Synaptic pruning is a fundamental process of neuronal circuit refinement in learning and memory. Accumulating evidence suggests that glia participates in sculpting the neuronal circuits through synapse engulfment. However, whether glial involvement in synaptic pruning has a role in memory formation remains elusive. Using newly developed phagocytosis reporter mice and three-dimensional ultrastructural characterization, we found that synaptic engulfment by cerebellar Bergmann glia (BG) frequently occurred upon cerebellum-dependent motor learning in mice. We observed increases in pre- and postsynaptic nibbling by BG along with a reduction in spine volume after learning. Pharmacological blockade of engulfment with Annexin V inhibited both the spine volume reduction and overnight improvement of motor adaptation. These results indicate that BG contribute to the refinement of the mature cerebellar cortical circuit through synaptic engulfment during motor learning.",
author = "Morizawa, {Yosuke M.} and Mami Matsumoto and Yuka Nakashima and Narumi Endo and Tomomi Aida and Hiroshi Ishikane and Kaoru Beppu and Satoru Moritoh and Hitoshi Inada and Noriko Osumi and Eiji Shigetomi and Schuichi Koizumi and Guang Yang and Hirokazu Hirai and Kohichi Tanaka and Tanaka, {Kenji F.} and Nobuhiko Ohno and Yugo Fukazawa and Ko Matsui",
note = "Funding Information: This research was supported by JSPS KAKENHI grants 16K18388, 18K06457, 18J00603 (to Y.M.M.), 18H05110, 19H03338, 20H05046, 20H05896, 22H02713 (to K.M.),16H06280, 18H05120, 19K22469 and 19H03323 (to Y.F.) by The Naito Foundation Grant (to Y.M.M.), by the Satoshi Ohmura Foundation (to Y.M.M.), by Technical support platforms for promoting research of Advanced Bioimaging Support JP16H06280 (to Y.M.M. and N.Ohono.), by Opto-Science and Technology, the NOVARTIS Foundation, Takeda Science Foundation (to K.M), by The Joint Usage/Research Program of Medical Research Institute (to T.A., K.T. and K.M.), by AMED under the Brain/MINDS project: JP21dm0207111 (to H.H.) and by a Columbia University Target of Opportunity award. We thank T. Maegawa (Fukui University), A. Imai and N. Hattori (NIPS) for their helpful technical assistance in EM data acquisition, N. Hosoi, A. Konno (Gunma University), K. Chen (Columbia University), T. Osakada and L. Weinhard (New York University) for their helpful technical discussion, WB. Gan (Peking University Shenzhen Graduate School) for critical reading of the manuscript and all the members of the Matsui lab. Funding Information: This research was supported by JSPS KAKENHI grants 16K18388, 18K06457, 18J00603 (to Y.M.M.), 18H05110, 19H03338, 20H05046, 20H05896, 22H02713 (to K.M.),16H06280, 18H05120, 19K22469 and 19H03323 (to Y.F.) by The Naito Foundation Grant (to Y.M.M.), by the Satoshi Ohmura Foundation (to Y.M.M.), by Technical support platforms for promoting research of Advanced Bioimaging Support JP16H06280 (to Y.M.M. and N.Ohono.), by Opto-Science and Technology, the NOVARTIS Foundation, Takeda Science Foundation (to K.M), by The Joint Usage/Research Program of Medical Research Institute (to T.A., K.T. and K.M.), by AMED under the Brain/MINDS project: JP21dm0207111 (to H.H.) and by a Columbia University Target of Opportunity award. We thank T. Maegawa (Fukui University), A. Imai and N. Hattori (NIPS) for their helpful technical assistance in EM data acquisition, N. Hosoi, A. Konno (Gunma University), K. Chen (Columbia University), T. Osakada and L. Weinhard (New York University) for their helpful technical discussion, WB. Gan (Peking University Shenzhen Graduate School) for critical reading of the manuscript and all the members of the Matsui lab. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = nov,
doi = "10.1038/s41593-022-01184-5",
language = "English",
volume = "25",
pages = "1458--1469",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "11",
}