Switching Between Two Types of Bursting Activity of Single Ca+2‐Activated K+ Channels in Dissociated Neurons

D. Yamamoto, R. D. Pinnock, D. B. Sattelle

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Single channel recordings of Ca2+‐activated K+ currents were made from dissociated cockroach neurons by means of the gigaohm‐seal patch‐clamp technique. Bursts of single channel openings were composed of two distinct classes: the ‘long‐open burst’ contained groups of long, rectangular, pulse‐like openings with durations of 3.5 to 1.2 ms (depending on membrane potential), whereas the ‘flickering burst’ consisted of clusters of brief openings with an average duration of 0.4 ms (voltage‐independent) separated by short closings with a duration of about 1.0 ms. The long‐open burst and the flickering burst appeared to reflect distinct states of a single Ca2+‐activated K+ channel because direct transitions between these two types of burst were often detected. We present a kinetic scheme for the gating activation pathway of a neuronal Ca2+‐activated K+ channel, based on these findings.

    Original languageEnglish
    Pages (from-to)89-94
    Number of pages6
    JournalJournal of Neuroendocrinology
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - 1989 Apr

    Keywords

    • calcium‐activated potassium channel
    • dissociated neuron
    • flickering channel
    • ion selectivity
    • patch clamp

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

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