Switch interactions control energy frustration and multiple flagellar filament structures

Akio Kitao, Koji Yonekura, Saori Maki-Yonekura, Fadel A. Samatey, Katsumi Imada, Keiichi Namba, Nobuhiro Go

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

Bacterial flagellar filament is a macromolecular assembly consisting of a single protein, flagellin. Bacterial swimming is controlled by the conformational transitions of this filament between left- and right-handed supercoils induced by the flagellar motor torque. We present a massive molecular dynamics simulation that was successful in constructing the atomic-level supercoil structures consistent with various experimental data and further in elucidating the detailed underlying molecular mechanisms of the polymorphic supercoiling. We have found that the following three types of interactions are keys to understanding the supercoiling mechanism. "Permanent" interactions are always maintained between subunits in the various supercoil structures. "Sliding" interactions are formed between variable hydrophilic or hydrophobic residue pairs, allowing intersubunit shear without large change in energy. The formation and breakage of "switch" interactions stabilize inter- and intrasubunit interactions, respectively. We conclude that polymorphic supercoiling is due to the energy frustration between them. The transition between supercoils is achieved by a "transform and relax" mechanism: the filament structure is geometrically transformed rapidly and then slowly relaxes to energetically meta-stable states by rearranging interactions.

Original languageEnglish
Pages (from-to)4894-4899
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number13
DOIs
Publication statusPublished - 2006 Mar 28
Externally publishedYes

Keywords

  • Bacterial swimming
  • Flagellin
  • Molecular dynamics
  • Supercoiling
  • Transform and relax mechanism

ASJC Scopus subject areas

  • General

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