Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models

Hiroto Nishino, Hannah M. Hollandsworth, Norihiko Sugisawa, Jun Yamamoto, Yoshihiko Tashiro, Sachiko Inubushi, Kazuyuki Hamada, Yu Sun, Hyein Lim, Siamak Amirfakhri, Filemoni Filemoni, Robert M. Hoffman, Michael Bouvet

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background/Aim: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. Materials and Methods: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. Results: Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm3 and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm3. The engraftment rate was 100%. Conclusion: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.

Original languageEnglish
Pages (from-to)3153-3157
Number of pages5
JournalIn Vivo
Volume34
Issue number6
DOIs
Publication statusPublished - 2020 Nov
Externally publishedYes

Keywords

  • Cell line
  • Colon cancer
  • Hepatocellular carcinoma
  • Liver metastasis
  • Nude mouse
  • Orthotopic
  • PDOX
  • Patient tumor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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